View Full Version : Desferal (DFO) Administration - HOW??

01-27-2006, 06:16 AM
Hi all :)

I would like to know if anyone has ever worked with Desferrioxamine (=desferal, DFO, DFX). Under hypoxic conditions, this substance activates HIF-1 and so EPO is produced.

The thing is that we have to inject genetically engineered C2C12 myoblasts treated with desferal into mice (I've read this has to be done intramuscularly) but I can't find how to do so correctly.

I read that it can be dissolved in PBS but I don't know how many injections nor how regularly I should inject. Could the dosis be 200-300mg/kg wt more or less? (I read this in the literature).

Besides, I am not used to doing intramuscular administrations in mice, so I would be very grateful if anyone could give me a hand with this issue as well.

I have read papers in which DFO has been used, but was administered alone (and I am interested in injecting cells treated with DFO, not DFO alone")

Thanks very much beforehand

11-19-2007, 01:01 AM
All I know on Desferioxamine is that:

Deferoxamine (also known as desferrioxamine, desferoxamine, DFO, DFOA or desferal) is a chelating agent (http://en.wikipedia.org/wiki/Chelating_agent) used to remove excess iron (http://en.wikipedia.org/wiki/Iron) from the body. It acts by binding free iron in the bloodstream and enhancing its elimination in the urine (http://en.wikipedia.org/wiki/Urine). By removing excess iron, the agent reduces the damage done to various organs and tissues, such as the liver (http://en.wikipedia.org/wiki/Liver).
source: http://en.wikipedia.org/wiki/Desferrioxamine

It is a medicine that binds to excess iron in the body. It is then excreted in the urine and faeces, thereby reducing iron levels in the body. Iron overload can also occur as the result of iron overdosage (iron poisoning). Desferrioxamine is given in this situation as an emergency transfusion. Desferrioxamine can also be used to bind to and remove aluminium. It is used in people on dialysis for end-stage kidney failure, with aluminium overload and aluminium-related bone disease.
Source: http://www.drugdelivery.ca/s33592-s-DESFERRIOXAMINE.aspx

Well, I hope that these will be of some help to others even though I am replying a bit late...

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