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06-03-2004, 01:37 AM
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chat transcript - biochemistry (mol. bio.)
21:22:54 [Step_1] in which phase does dna synthesis take place?
21:23:01 [acestep1] k 21:23:09 [acestep1] s phase 21:23:10 [crusher] S phase? 21:23:14 [DrMG] s phase 21:23:18 [Lorena] S 21:23:23 [Rahat3256] s phase 21:23:28 [Step_1] yes very good....off to a good start 21:23:32 [yamini] s phase 21:23:42 [acestep1] 21:24:09 [Step_1] which nucleic acids have 2 hydrogen and which have 3 hydrogen bonds 21:24:28 [DrMG] adenine and thiamine =2 21:24:32 [yamini] A 21:24:34 [DrMG] guanine and cystein = 3 21:24:39 [yamini] SORRY 21:24:47 kokushubila enters this room 21:24:55 [acestep1] agree with dr G 21:24:56 [Step_1] sorry, i meant the pairs like drmg said 21:25:00 [Lorena] C and G have 3 21:25:12 [yamini] A=T 21:25:16 [acestep1] np 21:25:26 [DrMG] am i allowed to ask questions too? 21:25:29 [crusher] AT and CG 21:25:35 [Step_1] yes, please do 21:25:43 [DrMG] k 21:25:43 [acestep1] sure 21:25:48 [yamini] C&G 21:25:49 [Step_1] yes crusher 21:25:51 [zeda] if cytosine is deaminated then what will be the result? 21:25:57 [acestep1] oops imean 21:25:57 [Lorena] yes, the more questions the better 21:26:05 [crusher] every one ask Q.or give ans.or just observe,right lorena/step1 21:26:06 [DrMG] tell me the chromatin structure 21:26:06 [Rahat3256] what will happen if we methylate the uracil 21:26:08 [acestep1] uracil 21:26:14 [Step_1] AT is 2 hydrogen bonds and GC has 3 21:26:15 [yamini] URACIL 21:26:19 [zeda] yes 21:26:24 [Lorena] we'll have uracil 21:26:30 [Step_1] thats right crusher 21:26:31 [Rahat3256] uracil tozeda 's answer 21:26:45 [acestep1] hey DrG didnt get u 21:26:49 [zeda] ok 21:26:49 [jnewdoc2b] URACIL BECOMES THYMINE 21:26:50 [Step_1] you will get thymine 21:26:52 [DrMG] i meant 21:26:55 [acestep1] u asking abt histones etc ? 21:27:04 [DrMG] what is makeup of a chromatin 21:27:06 [DrMG] like 21:27:14 [DrMG] i will give u the first answer 21:27:22 [DrMG] H2A 21:27:29 [DrMG] yes 21:27:32 [acestep1] k 21:27:37 [crusher] histone prories rapped around nucleosm.It can be combo of H2 H3 H4 (octamer) or H1 h2 H3 H4(condense) 21:27:51 [Step_1] drmg, can you use a brighter font color from the rainbow on the right....its hard to read the black 21:28:00 [acestep1] nono crusaher 21:28:04 [DrMG] a nucleosome is the histone 21:28:08 [DrMG] right? 21:28:17 [DrMG] k 21:28:32 [acestep1] h2,h3h4 octomer 21:28:33 [Step_1] H2A, H2B, H3, H4 histones 21:28:35 [zeda] ok,now very basic thing 21:28:35 [DrMG] ok 21:28:37 [DrMG] tell me 21:28:42 [DrMG] what is the charge of the histone 21:28:43 [acestep1] yes agree with step1 21:28:57 [yamini] POSITIVE 21:28:58 [zeda] correct writing of DNA sequence is always in...? 21:28:59 [acestep1] thsi is euchromatin - 10 nm 21:29:01 [DrMG] and the charge of dna 21:29:13 [DrMG] 5' - 3' 21:29:17 [crusher] 5-3 21:29:19 [Step_1] positive chare at physiologic ph 21:29:23 [acestep1] -ve 21:29:33 [zeda] yes 21:29:33 [acestep1] yes agree with crusher 21:29:50 [crusher] without H1 is 10nm and with H1 is 30 nm. 21:29:50 [zeda] 5' ( lt. side ) and 3'( rt. side) 21:30:03 [DrMG] dna = neg histone = pos 21:30:05 [acestep1] yes agree 21:30:07 [crusher] 7 21:30:09 [Step_1] sorry my ans was pos for the previous question, not for dna 21:30:09 [Roxanita] ok, back 21:30:14 [DrMG] what do u call the inactive chromatin 21:30:24 [Rahat3256] i will be back in 5 mins 21:30:32 [acestep1] 30nm 21:30:33 [yamini] HETEROCHROMATIN 21:30:35 [Lorena] heterochromatin 21:30:40 [acestep1] k rahat 21:30:50 [acestep1] yes agree 21:30:57 [DrMG] how does it look under microscope 21:30:57 [Step_1] agree 21:31:13 [jnewdoc2b] WHAT'S INVOLVED IN THE PROCESS OF HYBRIDIZATION? 21:31:14 [yamini] dense 21:31:15 [Step_1] fibre-like 21:31:17 [acestep1] condensed i think 21:31:17 [crusher] esy 21:31:21 [DrMG] lorena u r right 21:31:25 [Lorena] and euchromatin = true= active 21:31:25 [DrMG] yes ace 21:31:31 [DrMG] u r correct 21:31:32 [acestep1] probe n target dna 21:31:35 [Step_1] tightly packed for hetero 21:31:41 [DrMG] very good lorena 21:31:43 [jnewdoc2b] YES ACE 21:31:46 [acestep1] k 21:31:47 [DrMG] and step 21:31:56 [Roxanita] Eu=True 21:32:03 [acestep1] thnx Dr 21:32:13 [DrMG] 21:32:14 [Lorena] heterochromatin looks dark under the microscope 21:32:18 [zeda] what is the difference between B-DNA and Z-DNA? 21:32:27 [DrMG] what 3 things make a nucleotide 21:32:31 [acestep1] yes agree with lorena 21:32:48 [DrMG] yes i do too 21:32:56 [Roxanita] NB + Pentose+ Phosphate Group 21:32:58 [crusher] which wone is transcriptionally active hetero or Eu? 21:33:01 [acestep1] zeda- the right handed helix - nornal 21:33:02 [Step_1] left sided for B and right for Z 21:33:02 [jnewdoc2b] B IS RIGHT HANDED AND Z IS LEFT HANDED 21:33:04 [DrMG] eu 21:33:11 [Roxanita] Nitrogenous Base= Pentose= Nucleoside 21:33:18 [DrMG] agree wuth jnew 21:33:21 [Lorena] B DNA is left handed, B DNA is right handed 21:33:21 [crusher] nucleic acid roxanita? 21:33:35 [zeda] yes,jnew 21:33:38 [jnewdoc2b] HOW MANY BP/TURN AND WHICH DIRECTION IS AN A-DNA? 21:33:41 [zeda] u r right 21:33:42 [Lorena] i meant z is left handed 21:33:44 [nne] I agree 21:33:58 [acestep1] hey listen plz go slow 21:34:03 [acestep1] hehe 21:34:15 [Lorena] yes....one question at a time please 21:34:35 [acestep1] 21:34:39 [DrMG] ok 21:34:43 [yamini] 11bp/360 right handed 21:35:01 [acestep1] hey is this imp ? 21:35:01 [Step_1] i remember that z is left handed because that is what a straight line would look like if i drew it with my left hand 21:35:08 [Roxanita] what base pairs require higher meltin point? 21:35:15 [acestep1] 21:35:20 [DrMG] guanin and cysteine 21:35:21 [jnewdoc2b] I'M NOT SURE ABOUT THE 360. I HAVE IT'S 20 DEGREES AWAY FROM A HELICAL AXIS... 21:35:23 [Roxanita] good step 1 21:35:25 [acestep1] gc 21:35:31 [DrMG] cuz they have three bonds 21:35:32 [crusher] CG 21:35:38 [Lorena] the bases wthat contain cytosin and guanin 21:35:42 [Roxanita] that's right 21:35:42 [yamini] guanine cytosine 21:35:57 [zeda] good explanation step_1.Now.....factors which can denaturate DNA? 21:35:58 [acestep1] ok my q- wht is melting pt 21:36:02 [Lorena] good point 21:36:15 [crusher] high melting point 21:36:23 [Roxanita] what type of bond do base pairs have? 21:36:36 [Lorena] UV radiation, heat, chemicals 21:36:51 [zeda] at which H-bonds get seperated is melting point 21:36:58 [acestep1] ya but temp at which 50 %bonds r broken 21:37:01 [Roxanita] it's like melting the 2ble helix into a 2 singles separate strands 21:37:03 [zeda] yes lorena 21:37:07 [crusher] H bond roxinaya? 21:37:15 [Rahat3256] yah i am back 21:37:20 [Roxanita] they use it on DNA technology 21:37:29 [Roxanita] Hydrogen Bond right 21:37:31 [Lorena] welcome back rahat 21:37:34 [zeda] ? 21:37:49 [acestep1] yes roxanita 21:37:54 [Rahat3256] thank you lorena 21:37:55 [acestep1] 21:38:14 [jnewdoc2b] WHICH ONE IS MORE TIGHTLY WOUNDED A POSITIVE OR NEGATIVE SUPERCOILED DNA? 21:38:22 [Lorena] Tm is at the point 50% DNA is denatured 21:38:37 [acestep1] +ve 21:38:40 [Roxanita] we already pass cell cycle.... from the question.... 21:38:45 [zeda] ace..at what temp. 50% bonds r broken? 21:38:46 [acestep1] yes lorena 21:38:52 [Roxanita] which of protein sx occurs predominantly in S phase of the cell cycle? 21:38:55 [crusher] -ve 21:38:58 [yamini] positive 21:39:01 [acestep1] tht u have 2 see in teh graph 21:39:03 [Roxanita] which protein synthesis? 21:39:06 [jnewdoc2b] AGREE WITH ACE; IT'S POSITIVE 21:39:18 [Step_1] pos 21:39:25 [Lorena] replication? 21:39:32 [acestep1] k 21:39:49 [crusher] i thinkmits neg more tight 21:39:53 [Roxanita] hello...does anybody knows which protein sx occurs predominantly in S phase of the cell cycle? 21:39:55 [acestep1] yes agree with lorena 21:39:56 [Step_1] replication....agree 21:39:59 [yamini] DNA replication 21:40:13 [zeda] 58? 21:40:41 [Lorena] is that right rox? 21:40:59 [Roxanita] yup it's less than 60 c 21:41:07 [Roxanita] what answer? 21:41:17 [zeda] thanks rox 21:41:42 [Roxanita] Ok as nobody answers what is the protein sx that occurs predominantly in S phase of the cell cycle... 21:41:46 [jnewdoc2b] WHERE DO YOU SEE MULTIPILE ORIGIN OF REPLICATIONS? 21:41:54 [Roxanita] last chance.... 21:42:06 [yamini] prokaryotes 21:42:07 dsa503 enters this room 21:42:09 [Lorena] tell us rox, thae answer 21:42:21 [crusher] what is the bond b/w two base sugars 21:42:26 [Rahat3256] Eukaryotes 21:42:26 [Step_1] hi dsa 21:42:27 [acestep1] yes plz rox 21:42:28 [jnewdoc2b] ACTUALLY ITS EUKARYOTES, YAMINI 21:42:35 [zeda] hey rox..change this red color...hard to read 21:42:38 [crusher] eukaroytes 21:42:43 [acestep1] yes linear dna 21:42:43 [Roxanita] guys I didn't know this on my exam 21:42:44 [Step_1] the suspense is killing me 21:42:51 [Lorena] eukaryotes have multiple originas, prokaryotes have a circular chromosome 21:43:02 [acestep1] lol - step1 21:43:13 [dsa503] hello everyone 21:43:26 [Lorena] tell us tell us tell us 21:43:32 [Step_1] GAATTC and CTTAAG. what is this referred to as? 21:43:38 [Roxanita] the answer is "HISTONE" 21:43:39 [yamini] ok jnewdoc2b 21:44:02 [Roxanita] HISTONES S Phase 21:44:09 [yamini] palindrome step_1 21:44:13 [acestep1] codon n template? 21:44:17 [Rahat3256] cpmplimentary & antiparralal step 21:44:24 [zeda] ya...now nice 21:44:25 [Lorena] oh, good one rox!! 21:44:29 [Step_1] i didnt know that rox, even after i looked it up...thanks 21:44:37 [Roxanita] Remember: G1= "Growth", "Gap" 21:44:39 [zeda] nice Q 21:44:46 [Step_1] palindrome because it reads the same in the forward and reverse directions 21:44:47 [crusher] dna replication in 5-3 antiparrelel 21:45:03 [Roxanita] it was in atext book of a college in chicago 21:45:05 [acestep1] ic 21:45:08 [zeda] yes agree with crusher 21:45:19 [acestep1] oh ic 21:45:30 [acestep1] gd 1 rox 21:45:35 [crusher] plz slow down i,m not able to follow 21:45:38 [zeda] ok 21:46:05 [Rahat3256] step you were asking....... 21:46:32 [Lorena] palindrome is the asnwer to your questions step 1? i got lost 21:46:37 [Step_1] GAATTC and CTTAAG. what is this referred to as? but i already put the ans...so dont cheat if you saw it 21:46:41 [Roxanita] in what key phase the cell will make sure that none of the process have mistakes? 21:47:02 [Lorena] G1 21:47:08 [crusher] G2 21:47:12 [Roxanita] those are palindrome 21:47:14 [acestep1] g2 21:47:18 [Rahat3256] g2 21:47:27 [yamini] in which phase DNA repair takes place 21:47:36 [Roxanita] what is before Mitosis: G1 or G2...so remember 21:47:41 [yamini] G2 21:47:52 [crusher] again G2 21:47:57 [zeda] G2 21:48:03 [Rahat3256] G2 21:48:04 [Step_1] G2 21:48:08 [Roxanita] G2 21:48:10 [DrMG] which polymerase does the correction 21:48:18 [acestep1] agree 21:48:19 sweta_chandra enters this room 21:48:26 [Lorena] i'll remember that 21:48:27 [crusher] poly 1 21:48:49 [Roxanita] G2: repair of mismatches bases, yup 21:49:17 [Rahat3256] poly 1 & 3 21:49:20 [DrMG] i thought it was 111 21:49:23 [acestep1] ok wait b in eu n dna polymerase 1 for proof reading in prokary 21:49:24 [DrMG] i mean 3 21:49:32 [Step_1] repair is both G1 and G2....right? 21:49:32 [yamini] DNA REPAIR IN G1 21:49:33 [sweta_chandra] polymerase 1 does proof reading 21:49:34 [Roxanita] we are talking about the proofreading activity of DNA polymerase 21:49:40 julieog1 enters this room 21:49:45 [acestep1] oops yes dna poly 3 21:50:00 [DrMG] dna 1 removes rna primer 21:50:06 [DrMG] ? 21:50:19 [acestep1] yes 21:50:19 [crusher] poly 3 synthesis of leading and laging strand in pro 21:50:29 [DrMG] i agree with ace 21:50:33 [crusher] dna poly 1 remove Rna primer 21:50:36 [Lorena] agree with ace 21:50:50 [DrMG] it synethsize 5 -3 21:50:56 [DrMG] and proff reads 3-5 21:50:59 [acestep1] 21:51:26 [acestep1] yes syn always in 5-3 anti parallel n complentary 21:51:41 [DrMG] *proof 21:52:02 omwaseem enters this room 21:52:03 [Rahat3256] 3-5 exonuclease 21:52:07 [acestep1] but proof reading in 3-5 cuz it removes teh incorrect base as soon as it makes it which is at teh 3 end 21:52:20 [crusher] whts the ans of poly in repar 21:52:23 [DrMG] i agree 21:52:27 [acestep1] yes agree with rahat 21:52:31 [DrMG] it is 3 21:52:31 [Roxanita] G1 is a preparatory phase, prior to DNA sx, there is no repair 21:52:55 [Rahat3256] thank you ace 21:53:23 [acestep1] ur welcome any time 21:53:48 [zeda] what is telomerase activity? 21:53:54 adam enters this room 21:54:00 [acestep1] yes agree with rox 21:54:07 [DrMG] relieves supercoils 21:54:11 [Roxanita] in G1 the cell will make relevant proteins that will trigger the cell cycle to move forward. remember betwee G1 and S : Growth factors, including cyclins 21:54:31 [Step_1] agree, relieves supercoils 21:54:43 [acestep1] hmm . i think teh 5 end of teh new starnd is not made properly so teh new starnd will b smaller 21:54:46 [Lorena] repair the telomeres that otherwise woul,be lost in transcription 21:54:58 [yamini] ROXANITA CHECK OUT SECOND CHAPTER SECTION DNA REPAIR 21:54:59 [Rahat3256] agree with you 21:55:05 [zeda] yes 21:55:07 [Roxanita] one last...when will Gene expression occur, Phase? 21:55:42 [yamini] INTERPHASE 21:55:57 [zeda] normally it is present in.....embryonic cells,stem cells and in germ cells 21:56:07 [acestep1] yes agree with lorena 21:56:10 [DrMG] step 1 ...i think it is toipermerase 21:56:12 [Roxanita] Interphase has 3 subdivisions: G1-S-G2 21:56:16 [Lorena] agree with you zeda 21:56:20 [acestep1] n zeda 21:56:20 [DrMG] i have it mixed up with teleromerase 21:56:26 [Roxanita] even G0 21:56:44 [acestep1] interphase- rox 21:56:50 [Roxanita] what about it Yamini? 21:56:52 [zeda] which kind of cells have high telomerase activity? 21:56:57 [Lorena] is interphase the answer rox? 21:57:22 [sweta_chandra] eukaryotic cells have telomerase activity 21:57:22 [yamini] CANCER CELLS 21:57:31 [zeda] yes yamini 21:57:32 [Roxanita] yeah Interphase is global but specifically? G1 - S or G2 ? 21:57:33 [Step_1] oops, i mixed up the words, sorry 21:57:36 [Lorena] reproductive cells have hign telomerase activity(embryonic cells) and stem cells, also cancer cells does 21:57:36 [zeda] u r right 21:58:01 [Step_1] what polymerase is responsible for mitochondrial DNA in eukaryotes? 21:58:07 [DrMG] agree with lorena 21:58:11 [yamini] GAMMA 21:58:21 [Roxanita] in S phase we have the synthesis and on G2 we have it ready so the Gen expression will occur 21:58:29 [acestep1] agree with yamini 21:58:33 [Lorena] agree with yamini, gamma 21:59:01 [Step_1] dna polymerase gamma responsible for mito DNA is eukaryotes....good job! 21:59:15 [Roxanita] yup Gamma 21:59:27 [Lorena] you have the great questions rox 21:59:46 [Roxanita] Delta is for leaDing strand 22:00:01 [acestep1] yesagree 22:00:24 [Lorena] i guess then it is G2? rox? 22:00:25 [DrMG] agree 22:00:32 [Roxanita] Betta is for DNA repair 22:00:39 [yamini] ALPHA FOR LAGGING STRAND 22:00:41 [Roxanita] yup Lorena is G2 22:00:52 [Step_1] what are the stop and start codons? 22:00:53 [Roxanita] right yamini 22:01:19 [sweta_chandra] start are aug 22:01:21 [DrMG] aug.uga 22:01:28 [sweta_chandra] stop uaa, uag uga 22:01:34 [Lorena] stop: UGA,UAA, UAG, and start AUG 22:01:55 [Roxanita] <A target=new HREF="http://www.amazon.com/exec/obidos/ASIN/0071429484/qid%3D1085033910/sr%3D2-1/valuetheplace-20">First Aid</A>  22:01:59 [acestep1] agree 22:02:07 [Roxanita] Gosh. I meant F-irst A-id 22:02:22 [acestep1] np 22:02:29 [Step_1] AUG for start. UGA, UAG, and UAA for stop. mneuomonic is inAUGurate (start), and U Go Away, U Are Gone and U Are Away (stop) 22:02:33 [merjo13] Agree with Lorena 22:02:57 [Lorena] 22:03:02 [DrMG] thnx step 22:03:08 Rahat3256 enters this room 22:03:31 [Rahat3256] all of a sudden they said my chat session expired 22:03:37 [Roxanita] Types of RNA? 22:03:38 [Rahat3256] to get in to it again 22:03:42 [acestep1] yes gd 1 step1 22:03:45 [crusher] same wiuth me 22:03:58 [acestep1] awwwwwww. wb rahat 22:04:05 [acestep1] n crusher 22:04:05 [crusher] mrna tRNA, rRNA 22:04:10 [sweta_chandra] 3 types 22:04:14 [sweta_chandra] m, r, t 22:04:24 [DrMG] agree 22:04:25 [sweta_chandra] hn rna 22:04:26 [yamini] m-RNA,r-RNA,t-RNA 22:04:28 [nne] mRNA, tRNA rRNA 22:04:32 [Step_1] agree with crusher and sweeta mrt 22:04:41 [DrMG] which one is the most abundant 22:04:45 [Rahat3256] UGA...u go away/UAG...U are gone/UAA....U are away... 22:04:51 [Roxanita] wich one is the most abundant? 22:04:56 [DrMG] i mean largest 22:04:56 [Lorena] agree with sweta 22:05:09 [Step_1] r is most abundant (Rampant) 22:05:09 [dsa503] r rna 22:05:11 [Lorena] rRNA= rampant 22:05:12 [acestep1] rrna 22:05:15 [Rahat3256] you can remember the stop codons in this way 22:05:16 [DrMG] and which one the smallest 22:05:20 [sweta_chandra] m rna most abundant 22:05:21 [nne] rRNA 22:05:27 [dsa503] smallest is t rna 22:05:29 [Roxanita] good "rampan" 22:05:32 [DrMG] mrna is the largest 22:05:34 [Step_1] m is the largest (Massive) 22:05:44 [DrMG] u r right dsa 22:05:46 [acestep1] ok i think there were 3 more hn n sn n ribozymal rna 22:05:53 [Step_1] t is the smallest (Tiny) 22:05:53 [Roxanita] rampant 22:05:56 [Lorena] agree with step 1 22:06:24 [Rahat3256] me too 22:06:24 [acestep1] wow step1 22:06:28 [DrMG] agree 22:06:43 [nne] which one is the only one ranslated? 22:07:05 [Lorena] you are right ace , more RNA (hn, sn 22:07:07 [dsa503] m rna 22:07:09 [Roxanita] mRNA 22:07:11 [zeda] mRNA 22:07:13 [acestep1] m m rna 22:07:25 [Step_1] mRNA 22:07:36 [acestep1] thnx lorena 22:07:40 [Lorena] yes! 22:07:46 [nne] true 22:07:56 [Lorena] good nne 22:08:20 [dsa503] what are the types of rna polymerase? 22:08:36 [dsa503] & what do they synthesize? 22:08:59 [DrMG] I = rrna 22:08:59 [yamini] 1,2,3 22:09:07 [DrMG] II - mrna 22:09:11 [Roxanita] I - II- III 22:09:18 [acestep1] in pro only one abbprimer 22:09:18 [DrMG] III- trna 22:09:32 [acestep1] in eu there r 3 22:09:52 [zeda] which RNA participate in splicing? 22:09:54 [Roxanita] I --> rRna ; II--> mRNA ; III--> tRNA 22:09:55 [yamini] r-RNA,m-RNA,t-RNA respectively 22:09:55 [Lorena] RNAP 1: rRNA except 5s r RNA, RNAP2: hn RNA /mRNA and some snRNA; RNAP3:tRNA 22:10:17 [dsa503] 1 makes r rna 2 makes m rna hn rna & sn rna 3 makes 5s rrna & t rna 22:10:18 [Roxanita] with those exception that Lorena said 22:10:30 [Step_1] 1 makes rRNA, 2 makes mRNA, 3 makes tRNA 22:10:31 [Rahat3256] thanks lorena 22:10:36 [dsa503] good job lorena 22:10:44 [acestep1] agree 22:10:52 [Lorena] your welcome 22:11:03 [Step_1] RNT = 123 (rhymes ) 22:11:24 [Step_1] sorry RMT 22:11:30 [Roxanita] good 22:12:05 [Lorena] what polymerase is inhibited by the mushroom amanitin? 22:12:11 [dsa503] what diseases are caused due to DNA repair defects? 22:12:38 [zeda] II 22:12:38 [Roxanita] Xeroderma Pigmentosum... 22:12:48 [yamini] polymerase2 22:13:04 [Lorena] good zeda and yamini! 22:13:04 [acestep1] agree with both 22:13:10 [Step_1] DNA repair defects = Xeriderna Pigmentosum 22:13:11 [Lorena] ace too 22:13:30 [Lorena] yes, xeroderma pigmentosum 22:13:31 [crusher] poly 2? 22:13:32 [acestep1] thnx 22:13:50 [DrMG] thank u guys .this been very helpful...see u tomorrow 22:13:52 [yamini] HPNCC 22:13:53 [dsa503] also blooms syndrome & ataxia telangiectasia 22:14:06 [Lorena] thanks to you too Dr MG for the q's! 22:14:09 [Step_1] thanks for the great questions DrMG 22:14:28 [acestep1] ok for mushroom is it for both eu n pro 22:14:38 [Roxanita] Thymine Dimmer-Formation---> Xeroderma P. 22:14:44 [acestep1] yes agree with lorena n step1 22:14:55 [zeda] What is the function of GC rich region? 22:15:33 [dsa503] forms the gc loop 22:15:40 [Lorena] inhibits RNA 2 in eukaryotes ace 22:15:54 [crusher] its on 3 end....termination? 22:16:07 [zeda] yes 22:16:12 [Rahat3256] hey guys i have to go now 22:16:16 [Rahat3256] er mind 22:16:17 [zeda] termination of transcription 22:16:18 [crusher] form stem and loop 22:16:26 [Lorena] thank you rahat for being here today! 22:16:29 [acestep1] ok thnx lorena 22:16:37 [zeda] which region is recognised by RNA polymerase? 22:16:40 [acestep1] yes. take care rahat 22:16:45 [Rahat3256] wel come lorena 22:16:52 [Rahat3256] thank you ace 22:16:56 [Step_1] good seeing you rahat. 22:17:07 [crusher] TATA box 22:17:09 [acestep1] ur welcoem antime 22:17:25 [acestep1] ur welcome anytime imean 22:17:31 [nne] which of the followin enzymes may be the targets of a new drug that specifcally inhibits retroviral replication? DNA dependent DNA polymerase, RNA depenent DNA polymerase, DNA ligase, RNA polmerase, Topoisomerase II 22:17:55 [dsa503] topoisomerase 2 22:17:56 [Lorena] RNA dependant DNA polimerase 22:17:57 [zeda] promoter...-35 22:18:21 [acestep1] agree with lorena 22:18:23 [yamini] RNA DEPENDENT DNA POLYMERASE 22:18:27 [crusher] rna dep dna poly..reverse transcriptase 22:18:51 [Step_1] agree, rna dep dna poly 22:19:34 [Step_1]>[zeda] sorry didnt see question till now.....what was ans? 22:19:35 [crusher] promotor region tata box minus 10 in pro karo..DNA poly 1 22:19:39 [Lorena] good question nne 22:20:03 [nne] RNA dependent DNA polmerase synthesizes DNA from an RNA template and is essential for the replication of retroviruses but not for cells 22:20:51 [nne] Topoisomerases II relaxes supercoiled NA. 22:20:57 [Roxanita] good nne 22:21:15 [Lorena] 22:21:15 [crusher] which type of transcription occur in eukaryoutes ..monocistronic or polycistronic? 22:21:22 [nne] Supcoiled DNA. I mean 22:21:28 [Lorena] monocistronic 22:21:32 [acestep1] lol 22:21:42 [acestep1] agree 22:21:43 [yamini] MONOCISTRONIC 22:21:59 [Roxanita] agree 22:22:00 [dsa503] thanks nne good question 22:22:55 [Step_1] agree also 22:23:20 [Lorena] which of the following is an exact complementary copy of the template DNA? antisence RNA, primary transcript, mRNA, tRNA or hn RNA? 22:23:34 [Roxanita] eukaryotic genome has multiple sites of replication 22:24:09 [acestep1] hn rna i think 22:24:20 [acestep1] yyes agree with rox 22:24:27 [dsa503] hn rna 22:24:29 [crusher] i,m not sure about the ans... 22:25:01 [acestep1] ok wht does rifampin inh 22:25:12 [adam] Hi Guys 22:25:25 [acestep1] hi adam 22:25:33 [adam] rifampin inhibits the betaq subunit of RNA polymerase 22:25:42 [Step_1] hi adam 22:25:51 [Lorena] hi adam 22:25:52 [adam] Euk replication is Polycistronic 22:25:56 [yamini] prokaryotic polymerases 22:26:03 [acestep1] yes rna poly 2 22:26:14 [acestep1] of pro 22:26:34 [crusher] yes adam eukaryotic is polycistronic cos multiple genes are transcribed 22:26:40 [acestep1] oops imean prokaryote rna 22:26:57 [adam] Yes Crusher 22:27:02 [acestep1] gd job adam n yamini 22:27:16 [adam] Rifampin inhibits the beta subunit of RNA polymerase 22:27:19 [Step_1] agree, inhibit RNA polymerase 22:27:24 [acestep1] hey lorena whst the ans 2 ur q 22:27:26 [Roxanita] True or false? b) The leading strand runs 5’ to 3’ and is continuous 22:27:39 [Lorena] primary transcript is the direct product of transcription and has not been modified at all.It lacks poly adenylation and a 5'cap and still contains all the introns which will be spliced out later 22:27:51 [Roxanita] T o F ...c) Okazaki fragments are discontinuous and run 5’ to 3’ 22:27:57 [crusher] true 22:27:58 [adam] it binds to beta subunit of Prok. RNA polymerase 22:28:03 [Lorena] thats the aswer ace 22:28:12 [dsa503] true 22:28:24 [adam] B is true 22:28:27 [acestep1] thnx lorena 22:28:31 [crusher] true about okazaki 22:28:33 [adam] c is true 22:28:46 [Roxanita] Good guys 22:28:52 [acestep1] true for both - rox 22:29:03 [Roxanita] T o F e) The proofreading of the added nucleotides in between the lagging strands by DNA polymerase is performed 5’ to 3’ 22:29:10 [adam] I have a viral genetics q. may I ask? 22:29:13 [acestep1] oops the seond is false 22:29:20 [Lorena] false 22:29:24 [Step_1] yes please do adam 22:29:26 [sweta_chandra] false 22:29:27 [adam] e is false 22:29:28 [dsa503] false 22:29:33 [sweta_chandra] it is 3'-5' 22:29:45 [Roxanita] Guys you can always post your questions 22:29:47 [yamini] false 22:29:55 [Lorena] agree with sweta 22:30:05 [Roxanita] completely false 22:30:40 [Roxanita] The proofreading of the added nucleotides...This is the only process in DNA replication that is performed 3’ to 5’ 22:30:47 [Step_1] which mutation results in early stop codon? 22:31:06 [adam] in MRS they describe the RNA replication when an RNA virus enters the cell as being performed by cell RNA polymerase? is this true? ( i.e , does our RNA polymerase read RNA strands?) 22:31:12 [acestep1] non sense 22:31:15 [dsa503] nonsence 22:31:19 [adam] Thanx Rox 22:31:25 [sweta_chandra] yes agree 22:31:28 [Lorena] nonsence mutation 22:31:33 [yamini] nonsense 22:31:36 [Step_1] nonsense mutation ends in early stop codon...good job 22:31:57 [adam] Nonsense= makes no sense 22:31:59 [acestep1] yes i think they do 22:32:11 [adam] Missense= makes some sense 22:32:14 [Lorena] agree with ace 22:32:19 [Roxanita] In which of the following cell cycle phases do most cells spend the majority of their life?..M ; Go; G1; S; G2 ? 22:32:34 [Roxanita] that's a good one adam 22:32:45 [sweta_chandra] g0 22:32:48 [acestep1] Go 22:33:01 [Lorena] i know G0 for neurons and muscle cells..... 22:33:04 [adam] G0 22:33:08 [nne] RNA processing can be best describe by: Occurs in Cytoplasm; Reslts in the formation of new covalent bond btw RNA and DNA ; inludes the methylation of nucleotides; results in the addition of nucleotides to the primary transcript of rRNA; includes the addition of a tail of polyadenylic acid 22:33:13 [Roxanita] completely right guys 22:33:13 [dsa503] adam what is th ans to your q 22:33:15 [Step_1] good 1 22:33:16 [Lorena] it is the same for the rest of the cells? 22:33:49 [adam] I don't really know, It is rather puzzling for me and was wondering if any body knows? 22:33:50 [nne] Polyadenylic acid in the 5' end 22:35:12 [adam] a retroviruses was allowed to infect an expermental cell, where does Reverse transcription occurs? cytoplasm, nucleus? 22:35:21 [Roxanita] uhm the last one? 22:35:29 [Lorena] i think positive sence RNA virus are dependent of our RNA polymerase adam 22:36:18 [crusher] cytoplasm 22:36:25 [dsa503] nucleus..? 22:36:30 [sweta_chandra] i think cysm too. 22:36:33 [yamini] cytoplasm 22:36:54 [Roxanita] cyotplasm 22:37:03 [adam] Cyto true 22:37:06 [acestep1] agree with all 22:37:57 [sweta_chandra] which aa is deficient in cystic fibrosis? 22:38:04 [nne] Anybody know the answer to nne's question? 22:38:23 [acestep1] phenylalanine 22:38:30 [adam] Lorena, how about the negative RNA. how does it replicate using our RNA polymerase? or they use RNA polymerase for both transcription and replication? 22:38:32 [acestep1] i think nne its A 22:38:39 [sweta_chandra] yes correct ace! 22:38:50 [adam] phenyalanine 22:39:02 [acestep1] thnx sweta 22:39:25 [nne] I think A is wrong 22:39:41 [yamini] phenylalanine 22:39:51 [acestep1] i think -ve r first coverted 2 + ve rna n then they r translated 22:39:51 [dsa503] e sounds right too 22:39:56 [crusher] deletion of phenylalanine at position 508 position 22:39:58 [Lorena] negative sence , they have their own polymerase 22:40:03 [Roxanita] Which of the following substrates is used in both purine and pyrimidine biosynthesis?a) Aspartate b)Glycine c)Glutamine d)Carbamoyl phosphate 22:40:07 [adam] what is the role of aflatoxin in hepatocellular carcinoma? 22:40:40 [crusher] glycine is it 22:40:49 [adam] true lorena but when they are copied into mRNA, they use our RNA polymerase for replication according to MRS! 22:40:56 [acestep1] agree with lorena 22:41:23 [crusher] aflatoxin destroy our hepatocytes 22:41:30 [sweta_chandra] aflatoxin is a chemical carcinogen 22:41:33 [adam] I am refering to MRS page 168 figures 22:41:40 [yamini] carbamoyl phosphate 22:41:46 [dsa503] Carbamoyl phosphate 22:42:18 [Lorena] i dont know adam 22:42:20 [adam] crusher, does this mean aflatoxin cause hepatitis? 22:42:22 [crusher] about -ve and =ve sense rna given very good with diagram in k,,a,,p,,l,,an micro 22:42:43 [Roxanita] Carbamoyl phosphate and aspartate are the only 2 substrates directly used to synthesize pyrimidines. Aspartate, N10-Formyltetrahydrofolate, glutamine, glycine and CO2 are sources for purine synthesis. 22:42:46 [acestep1] k thnx crusher 22:42:53 [adam] I have no K a p l a a n micro notes 22:43:05 [Roxanita] answer: aspartate for both 22:43:20 [Lorena] thank you roxanita 22:43:51 [adam] Which nucleotide bases require Folate for their synthesis? 22:43:51 [crusher] good Q roxanita 22:44:08 [crusher] uraciul is it? 22:44:20 [dsa503] thymine 22:44:39 [yamini] purines 22:44:48 [dsa503] oh 22:44:51 [Step_1] thymine i think 22:44:57 [acestep1] thymine i think 22:45:04 [acestep1] hehe 22:45:30 [Step_1] you read my mind 22:45:56 [sweta_chandra] yes purines 22:46:25 [acestep1] lol 22:46:30 [yamini] &thymine 22:46:47 [acestep1] ok y r dna _ve charged 22:46:48 [adam] Purines ( adenine+ guanine) + Thymine 22:47:03 [crusher] what is bond between the two ribose or deoxyribose sugars(real Q) 22:47:05 [acestep1] k 22:47:23 [sweta_chandra] phosphodiesterase bond 22:48:00 [acestep1] h bond on alternate n phosphodiester on teh smae starnd 22:48:15 [acestep1] oops imean same strand 22:48:18 [dsa503] phosphodiester bond 22:48:29 [adam] which group of people are naturally resistant to treatment with Azathioprin? 22:48:44 [crusher] yes it is phosphodiesterase bond 22:49:23 [Step_1] agree phosphodiesterase bond according to kap lan 22:50:03 [adam] is there a bond called phosphodiesterase? u guys mean phosphodiester, right? 22:50:45 [acestep1] yes my mistake the h bonds r b/w bases of the nucloetides 22:50:53 [Roxanita] Which of the following is not true concerning fungal RNA processing? A)It occurs in the cytoplasm b)The initial product of transcription is called heterogeneous RNA. C)The 5’ end is capped with a 7-methyl-G d) A polyadenosyne tail is synthesized on the 3’ end e) Introns are spliced out 22:51:31 [Step_1] sorry, i meant phosphodiester 22:51:49 [acestep1] b? 22:52:03 [adam] is it C Rox? 22:52:04 [crusher] B 22:52:04 [Lorena] i would guess b too 22:52:08 [yamini] b) 22:52:11 [acestep1] actually except a they all sound false 22:52:28 [Roxanita] this is a funny one.... 22:52:44 [Roxanita] Because Fungi are Eukaryotes. The entire process occurs in the nucleus. The 5’ end cap and the polyadenosyne tail are believed to stabilize the structure for protein synthesis. 22:52:57 [nne] adam, what's the ans t your question? 22:53:11 [acestep1] OmG rox tht was a gd 1 22:53:21 [Roxanita] is from Web Master 22:53:34 [acestep1] ic 22:53:36 [Lorena] very good rox! 22:53:46 [acestep1] 22:53:57 [adam] People with Lysch Nhyan syndrome. a USMLE favorite! 22:54:00 [dsa503] good one rox 22:54:11 [acestep1] k 22:54:20 [nne] Ok 22:54:27 [Roxanita] Which of The following pairs of aa are found in large quantities in the region of DNA bound to histones? A)Arginine, lysine b) Aspartate, glutamate c) Tyrosine, serine d) Phenylalanine, praline e) Glycine, alanine 22:54:42 [dsa503] wow adam your q's are great!! 22:55:11 [Lorena] arginine and lisine 22:55:13 [dsa503] Arginine, lysine 22:55:16 [yamini] A 22:55:23 [acestep1] a i think 22:55:23 [adam] arginine , lysine 22:55:45 [Lorena] they give histones their positive charge 22:56:03 [acestep1] yes agree with lorena 22:56:06 CARIBMD enters this room 22:56:13 [Step_1] arginine and lysine 22:56:25 [acestep1] hey lorena ur v gd at mol ;0 22:56:26 [Step_1] hi carib 22:56:33 [Roxanita] good Adam because... DNA is a (-) charged molecule and would bind to (+) charged histones, which are the basic aa (arginine, lysine) at physiological pH. 22:56:38 [acestep1] 22:56:39 hutals exits from this room 22:56:41 hutals exits from this room 22:56:42 [Roxanita] I meant good everybody 22:57:03 [adam] 22:57:22 hutals enters this room 22:57:35 [Lorena] thank you ace 22:58:08 [acestep1] . no seriously 22:58:43 [Step_1] yes lorena....i wish i knew this stuff half as well as you. 22:58:49 [adam] how does TCA cycle contribute to protein synthesis? 22:58:51 [Step_1] 22:59:00 [Lorena] are you guys kidding me?? 22:59:01 [acestep1] 22:59:18 [acestep1] nooooooooooooooo 22:59:31 [Roxanita] Let's talk about .........wait a minute........how Adam? 23:00:06 [Step_1] i've always stuggled with mol biol, but you seem to know you're stuff 23:00:16 [acestep1] ya how adam 23:00:34 [acestep1] 23:01:09 [Lorena] 23:01:21 [Lorena] yes, how adam 23:01:23 [acestep1] hey guys listen b/f we all log off i have a request 23:01:26 [adam] It makes GTP, remember? GTP, used in binding of tRNA to the ribosome during translation 23:01:52 sweta_chandra enters this room 23:01:54 [acestep1] wow adam tht was good 23:02:06 [Step_1] good one 23:02:08 [adam] 23:02:12 [Lorena] wow....very good!!! 23:02:15 [Roxanita] Thanks Adam, good1 23:02:18 [acestep1] 23:02:40 [Step_1] whats your request ace? 23:02:47 [acestep1] hey guys listen i need a study partner 23:02:52 [crusher] great adam 23:03:00 [adam] excessive consumption of which sugar ( disacchride ) may cause Hyperuricemia? 23:03:06 [acestep1] im having diff conc 23:03:12 [Step_1] i thought we were all your study partners 23:03:24 [acestep1] lol 23:03:28 [Lorena] yes, we are your study partners! proud to be!! 23:03:39 [acestep1] yes definitely but on a daily basis as well 23:03:49 [adam] cheer up 23:03:58 [nne] i am available 23:04:01 [Lorena] you mean online? 23:04:01 [acestep1] hey thnx lorena 23:04:06 [Roxanita] Which of the following is not true regarding RNA Polymerase? a) RNA polymerase I synthesizes rRNA b) RNA polymerase II synthesizes mRNA c) RNA polymerase III synthesizes tRNA d) Alpha-amantin inhibits the RNA polymerase complex e)Prokaryote RNA polymerase makes rRNA, mRNA and tRNA. 23:04:07 sana123 enters this room 23:04:09 [acestep1] yup 23:04:42 [acestep1] soem personal probs n i have 2 take my exams by august 23:04:43 [crusher] e is wrong 23:04:44 [adam] d. I think its Beta not alpha domain 23:04:54 [Step_1] i think we should all use the forum on daily basis to ask and ans questions about whichever subject we're on. roxanita provided a bunch of good ones this week, but not many of us were around to discuss 23:05:10 [Lorena] d 23:05:13 [acestep1] yes v true 23:05:21 [Roxanita] yup I did 23:05:29 [adam] I don't know that ya guys come here every day 23:05:47 [adam] so, what is the answer Rox? 23:06:00 [Roxanita] I will post my questions 2004 23:06:24 [acestep1] d i think 23:06:25 [Step_1] that would be great rox....thanks 23:06:29 [yamini] D 23:06:35 [nne] I agree with step 1 23:06:36 [acestep1] yes thnx Rox 23:06:37 [Roxanita] Oops....yup is d) 23:06:44 [adam] cool 23:06:46 [Roxanita] Good guys, all great 23:06:58 [acestep1] no adam we meet twice a week buit we put up soem qs on teh forum 23:07:00 [adam] so, Rox, you the one who puts the questions? 23:07:11 [nne] pls contact e too 23:07:20 [Roxanita] ok now Glycogen Storage Disease.... 23:07:24 [Lorena] sure 23:07:36 [acestep1] wait thsi was for sunday? 23:07:51 [Roxanita] I am but don't tell anybody b/c the administrador gets upset if I write 23:07:53 [adam] what is all this contact about, did I miss any thing? 23:08:18 [adam] : No Body ME tell 23:08:20 [acestep1] lol 23:08:23 [Roxanita] 23:08:40 [Lorena] lol 23:08:41 [Step_1] rox will post the questions in the forum and we can all stop by in between chats everyday to give input and solve together....sound good 23:08:49 [yamini] FRUCTOSE CAUSES HYPERURICEMIA ADAM 23:08:49 [acestep1] k 23:08:59 [crusher] lol,,,yes 23:09:07 [acestep1] yes 23:09:08 [adam] when? where? how? i am lost 23:09:14 [Roxanita] 23:09:28 [adam] excellent Yamini thought ya guys forgot about it 23:09:37 [Roxanita] what about the Histone synthesisi, we didn't know and what about the Bioenergetic stuff 23:10:04 [adam] Rox! what are ya talkin about? did I miss any thing? 23:10:09 [Step_1] no set time since its everyday, just stop by when you can in the step 1 forum of valuemd. we can solve together. then we will still meet as scheduled on wed and sat at 9pm for live chats 23:10:26 [Lorena] yes 23:10:27 [Roxanita] 23:10:36 [acestep1] k 23:10:46 [yamini] OK 23:10:52 [Roxanita] The majority of cellular proteins and RNA are synthesized throughout the cell cycle and are only interrupted briefly during M phase. Synthesis of which of the following proteins occurs predominantly in S phase of the cell cycle? 23:11:28 [adam] Histones 23:11:51 [Roxanita] well, I didn't know that 23:11:55 [Roxanita] Cyclins 23:11:57 [yamini] histones 23:12:03 [Step_1] histones from earlier answer i think 23:12:04 [Roxanita] Oops 23:12:16 [Lorena] guys i have to go 23:12:37 [Lorena] i will see you in the next chat , i am so happy with this group !! 23:12:40 [acestep1] hey lorena take care 23:12:43 [Roxanita] No Lorena don't go 23:12:45 [adam] what Rox? 23:13:02 [yamini] good night lorena 23:13:06 [Step_1] thanks for the excellent chat lorena. i learned alot from you tonight coz i'm terrible at biochem....thnx 23:13:10 [acestep1] yes . all of us r glad 2 b with u 23:13:13 [Roxanita] no, no its fine your answer adam 23:13:27 [Lorena] bye!!! 23:13:27 [adam] Histones? 23:13:34 [adam] Bye lorena and thans 23:13:35 [Step_1] bye 23:13:38 [adam] thanks 23:13:50 [acestep1] byee 23:14:30 [crusher] bye lorena thanks for great information provided 23:14:42 [adam] is it cyclins or histones Roxan? 23:14:52 [Roxanita] Bye Lorenita 23:15:02 [Roxanita] HISTONES Adam 23:15:05 [adam] By Lorenaaaaaaaaaaaaaaaaaaaaaaaaaaaa 23:15:06 [Step_1] next time is metabolism section of biochem, but what else? is there any other section we missed for tonight? 23:15:13 [adam] nk Roxannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnit 23:15:30 [acestep1] hey we ddint discuss the pt mutations 23:15:35 [yamini] genetics 23:15:35 [Roxanita] Adam there was a question on Bioenergetic 23:15:40 [acestep1] n also the ad n ar d/s 23:16:18 [acestep1] also abt how we syn of dna n rna 23:16:20 [Roxanita] we are still here so we can keep going right? 23:16:27 [acestep1] like teh enzymes etc 23:16:29 [adam] yes Roxy, shoot! but don't kill me 23:16:36 [acestep1] ya rox 23:16:41 [crusher] we shall continue 23:16:42 [adam] no left, not right! 23:16:43 [acestep1] lol 23:16:49 [Roxanita] I am just saying that in the real exam they come 23:17:02 [adam] yeah and they go too 23:17:03 [acestep1] ya also pcr n sountern bot etc r lft 23:17:06 [Step_1] yes, i was just wondering which sections are fair game for tonight and which to skip for next time 23:17:20 [sana123] hi everybody,i am new in this chat and am not able to see the whole of the chat session u guy did today,does it come in pages to which we need to know links or what 23:17:20 [Roxanita] what do you want to do guys....step 1? 23:17:20 [crusher] roxanita ur Q r awesome....keep askin 23:17:56 [adam] what was the bioenergetic q Rox? 23:18:01 [crusher] although i knew the ans of very few 23:18:06 [Step_1]>[sana123] hi sana, i'll post the chat in the step 1 forum at the end 23:18:08 [yamini] what is the effect of non-competitive inhibitor on km &Vmax? 23:18:21 [acestep1] no sana step1 will post todays session on teh forum i think 23:18:25 [adam] is it aclculations or about stuff with positive and negative values? 23:18:38 nne enters this room 23:18:39 [Roxanita] thanks but we can push ourselves by trying to solve the questions, everybody should put questions. Just 23:18:41 [crusher] its effect on vmax only n it dec vmax 23:18:43 [sana123] and roxanita, i would like to ask a few things about the q u posted regarding bioenergetics 23:19:12 [acestep1] k . thnx 23:19:15 [Roxanita] Enthalpia, Entropia, Free Energy changes, free Std energy change 23:19:27 [adam] 23:19:33 [acestep1] hey guys ill b back in 20 mins im really tired 23:19:52 [acestep1] lol 23:20:04 [Step_1] no prob ace 23:20:06 [nne] i don't understand this lineweaver burke thing 23:20:32 [Roxanita] I am lost on this Yamini, can you explain please? 23:20:33 [acestep1] thnx step1 23:20:54 [adam] wow, what about them Roxy? this sounds a tough one! Sh. 23:21:13 [Roxanita] 23:21:15 [Step_1]>[nne] i think there is a good question with explanation and pics on the forum somewhere. i can look it up for you and provide the link tomorrow if you want 23:21:32 [adam] 23:21:40 [adam] 23:21:48 [sana123] while adding the standard free energies of the cosecutive reactons we have to look at the sign which comes at the ebnd,either its is + or -,the reaction will proceed if its - and will not if its +,but this doesn't explain why z' option will accumulate most at equilibrium,would u or anybody plz expl 23:21:52 [Roxanita] Yamini, about your Q) effect of non-competitive inhibitor on km &Vmax? 23:21:53 [yamini] Km-no change,Vmax decreases 23:22:26 [Roxanita] it's like ALgebra, very fun 23:22:44 [kokushubila] Hi guys, I was Dead in this stuff , THANKS TO YOU ALL I am now ALIVE 23:23:05 [adam] yep, Km no change and V max decrease 23:23:09 [Roxanita] thanks yami 23:23:19 [sweta_chandra] v max increases or decreses? 23:23:27 [Roxanita] I remember that from pharm 23:23:27 [adam] welcome back to life Kokush 23:23:39 [adam] V max decreases 23:23:46 [sweta_chandra] 1/vmax decreases i guess 23:24:07 [Step_1] good kokush....i'm still trying to revive myself 23:24:11 [crusher] dec vmax 23:24:40 [sweta_chandra] 23:24:40 [adam] wassup Rox? Shoot! 23:24:51 [Roxanita] ΔG0′ : V ↔ W : - 4.0 ------- W ↔ X : +0.4 --------- X ↔ Y : – 3.4 ----------Y ↔ Z : + 5.7 23:25:17 [adam] v-w 23:25:23 [Roxanita] the question was From the values of ΔG0′ given in the table, which compound in the overall metabolic pathway V ↔ W ↔ X ↔ Y ↔ Z will accumulate in the largest amount at equilibrium? 23:25:40 [adam] i donno how to do these signs Roxan 23:25:58 [Roxanita] do you have Lippincott's Biochem? 23:26:09 [adam] who? me? 23:26:12 [Roxanita] that was a question 23:26:21 [Roxanita] yeah you... 23:26:35 [adam] yes, I do 23:26:48 [Roxanita] we guys have to start reading these stuff because it seems <a target=new href="http://click.linksynergy.com/fs-bin/click?id=c97WUMRO5hY&offerid=47491.10000058&type=3 &subid=0" >Kaplan</a> doesn't cover all themes 23:26:58 [Roxanita] I meant K-aplan 23:27:13 [adam] the answer to ur question is W 23:27:33 [Roxanita] I think it's Z 23:27:43 [crusher] plz explain wht ita all about 23:27:56 sana123 exits from this room 23:28:07 sana123 enters this room 23:28:20 [adam] I think there is a similar q in Lippincott's . isn't it? 23:28:29 [Roxanita] the original question comes in a table...they give you the following reactions and their ΔG0′ 23:29:16 [adam] the one with the lowet delta G is the fastest 23:29:27 [Roxanita] just apply the rule of: ΔG0′ of two consecutive reactions are additive 23:29:29 [adam] because it needs the lowet energy to proceed 23:29:55 [Step_1] k.aplan isn't as good of reference as lippencott, but its better for high yield stuff 23:30:08 [Roxanita] the (-) means it goes to the right direction: loss of free energy A-->B 23:30:35 [Roxanita] I love k-aplan but we have to cover those themes that are not there like this one 23:30:59 [Step_1] i agree 23:31:04 [adam] so, what would be the answer to ur Q rox? 23:31:41 [Roxanita] if you put the reactions in columns you will take off Ws, Xs and Ys 23:32:24 [Roxanita] and then you just have V --> Z if you do the summatory you will have a negative value which means it will accumulate to the right what is Z 23:32:52 [Roxanita] it's like algebra, you just eliminate same values at both sides, remember? 23:33:07 [Roxanita] so , following these for me it would be Z 23:33:15 [Roxanita] What do you think? 23:33:47 [Roxanita] anybody Is that bad? 23:34:05 [Roxanita] hellooooooooooooooooo 23:34:14 [adam] yes, 23:34:18 [adam] i am with ya 23:34:26 [adam] just trying to make the summation 23:34:31 [Step_1] i think thats right 23:34:50 [sana123] but u know what rox,the y to z has +5.7,why would it accumulate the most at equilibrium 23:34:56 [Roxanita] in the book they have an example similar but only 2 reactions 23:35:14 [adam] so, you mean z is produced the most? 23:35:48 [Roxanita] it doesn't matter the values just make the summatories, because they will eliminate 23:36:26 [adam] 4-4=0, will eliminate 23:36:29 [Roxanita] the summatory is negative it means ---> 23:36:38 [Roxanita] uhu 23:36:50 [adam] but 5.7-3.4 is not equal zero, how would they eliminate? 23:37:57 [Roxanita] V ↔ W – 4.0 ; W ↔ X +0.4 ; X ↔ Y – 3.4 ; Y ↔ Z + 5.7 23:38:03 [adam] which page is the example u rfered to? 23:38:35 [Roxanita] write: V+W+X+Y = W + X+ Y+ Z 23:38:54 [Roxanita] V+W+X+Y ---> W + X+ Y+ Z 23:39:01 [Roxanita] what left there? 23:39:17 [Roxanita] V --> Z right? 23:39:38 [hutals] makes sense 23:39:39 [sana123] v to z yes 23:39:43 [Roxanita] page 64 Lippincott's 23:39:44 [adam] HUMMMMMMMMM 23:40:03 [adam] the glucose example? 23:40:08 [Roxanita] now separate make the summatory and what you got, doesn't matter, you just know it's negative 23:40:19 [sana123] and since product comes -1.3 it will accumulate the most 23:40:44 [Roxanita] yes, they cancel glucose 6-P b/c it's in both sides 23:40:49 [sana123] u r genious rox,thanks,it was badly stuck on my mind 23:41:00 [Roxanita] since it is - value, it goes to the right --> 23:41:05 [Roxanita] who is on the right side? 23:41:13 [Roxanita] A --> Z 23:41:30 [Roxanita] sorry V --> Z 23:41:43 [Roxanita] Does it make sense guys? 23:42:01 [sana123] thanks rox,its makes good sense 23:42:10 [hutals] that was close, i was wondering where the A came from 23:42:29 [hutals] thnx for explaining that rox 23:43:16 [sana123] Q-â-Hexosaminidase is made up of á and â subunits. Tay-Sachs disease is due to a mutation in the á-subunit gene, and Sandhoff disease is due to a mutation in the â subunit gene. If a carrier of Tay-Sachs disease marries a carrier of Sandhoff disease, what is the probability that their first child w 23:43:28 [Roxanita] ok, for everybody, if ΔG0′=0 A<>B are at equilibrium; ΔG0′<0 A-->B right direction; ΔG0′>0 A<--B left direction 23:43:37 [adam] zero 23:44:08 [sana123] will have either disease? 23:44:17 [yamini] zero 23:44:58 [adam] so Rox, your answer would be z? 23:45:13 [Roxanita] yes Z 23:45:33 acestep1 enters this room 23:45:33 [adam] what was the options? were they like v, w, x, y or z? 23:45:39 [Roxanita] 23:45:58 [adam] hosssssshhhhhhhhhhhhh 23:45:59 hutals exits from this room 23:46:02 [Roxanita] V; W; X; Y; Z 23:46:05 hutals exits from this room 23:46:20 [adam] that make sense now 23:46:34 [Roxanita] 23:47:00 hutals enters this room 23:47:12 [adam] hummmmmmmmmmmmmmm. okay. are we safe here? 23:47:17 [Roxanita] the probability is Zero Sana? 23:47:26 [Roxanita] I think so 23:47:34 [adam] p) 23:48:04 [adam] 23:48:06 [Step_1] 23:48:26 [Roxanita] Uhm sorry STEP 1 23:48:30 [adam] 23:48:56 [sana123] thnaks rox, i neded to confirm the right ans 23:49:02 [Step_1] 23:49:12 [adam] Roxy yeah, there is a table on page 64 that refers to the directions 23:49:22 [Roxanita] I am asking you 23:49:33 [adam] who? me? 23:49:59 [Step_1] anything else high yield that we should go over? 23:50:09 [Roxanita] as I told you guys those question from N came on the real thing for me don't miss those themes 23:50:28 [adam] yes Rox! Lets Rock(x) and role 23:50:38 [adam] okay 23:50:43 [Roxanita] ok guys, kind of tired, but good information. Thanks step 1 for your great graphics, as everybody saw on the forums 23:50:57 [adam] did u do take ur exam rox? 23:50:58 [yamini] recombinant DNA WE Didnot discuss 23:51:21 [Roxanita] Adam, great to have you here 23:51:38 [adam] step1? do u have graphics on the froum? which graphs 23:51:44 [Roxanita] go ahead yamini, you know a lot share a little with us 23:51:50 [adam] Thanks Rox. 23:51:57 [adam] do u come here every day? 23:52:12 [adam] do u gusy have this discusion every day? 23:52:29 [Step_1] i posted some pics to help with explanations when i ans some of the questions on the forum...i'm a visual learner 23:52:33 [Roxanita] go to check the chedule for chat group on the forums 23:52:47 [adam] Roxy? how can I catch up with you in ur chats? 23:52:52 [Roxanita] schedule 23:53:12 [yamini] what is the enzyme required for PCR? 23:53:33 [adam] Taq DNA polymerase 23:53:50 [Step_1] http://www.valuemd.com/mycalendar.php for calendar and http://www.valuemd.com/viewtopic.php?t=18530 for table of schedule 23:54:28 [Roxanita] yup Taq polymerase 23:54:40 [Step_1] i agree 23:54:47 [adam] yeah great Roxy 23:55:06 [yamini] YES,roxanita&adam 23:55:21 [adam] yes Yamini, next q 23:55:49 [Step_1] which genetic testing for DNA? RNA? Protein? 23:56:07 [adam] DNA....Southern 23:56:12 [adam] RNA... NOrthern 23:56:18 [adam] Protein...WEstern 23:56:26 acestep1 enters this room 23:56:31 [yamini] in which type of BLOT gel electrophorosis is not required? 23:56:39 [adam] next yamini 23:56:43 [Step_1] DNA is Southern, RNA is Northern, Protein is Western.....good job adam 23:57:13 [acestep1] hey guys sorry my net is giving em probs 23:57:31 [adam] what is wouthwestern blot? 23:57:39 [Step_1] Dot Slot is not required 23:58:06 [yamini] yes,step_1 23:58:34 [adam] sorry, what is Dot slot? 23:58:37 [acestep1] ita test for proteins 23:58:49 [Step_1] Southwestern is to analyze DNA-protein interaction 23:58:56 [Roxanita] Northern Blot? 23:59:07 [adam] good step 1 23:59:12 [acestep1] test for anything dna , rna proteins etc 23:59:15 [sana123] Fragile X Synd. is diagnoses by using what kind a blot? a:Northern b:western c:southern 23:59:18 [adam] what about Northern blot rox? 23:59:45 [yamini] dot blot is used to study RNA,DNA,PROTIENS 23:59:46 [adam] i think it whould be southern! 23:59:53 [acestep1] northern is rna 23:59:53 [Step_1] Dot (slot) is a type of blot to analyze DNA, RNA, or protein. it doesn't require electrophoresis 00:00:14 [adam] so, is the only difference is that it doesn't need gel electropheresis? 00:00:17 [yamini] FRAGILEX-NORTHERN BLOT 00:00:23 [acestep1] i think southern blot- sana 00:00:39 [acestep1] oops yes yamini si right 00:00:44 [Step_1] the only major different that we should know at least 00:00:44 [sana123] yes yamini u r right, its orthern blot 00:00:54 [adam] why sana? 00:01:08 [adam] why it is northern? 00:01:13 [acestep1] sountern is for myotonic dystrophy 00:02:02 [acestep1] they see teh amt / length of rna in each tissue 00:02:07 [Step_1] what screeni     
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06-03-2004, 01:46 AM
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chat transcript - biochemistry (mol. bio.) cont.
00:02:07 [Step_1] what screening test for HIV? 00:02:15 [adam] why southern for Myotonic dystrophy? 00:02:21 [sana123] the basic idea behind using nothern is to analyse Rna extracted from a tissue to determine what genes are being expressed, and which tissues express the FMR1 gene involved in fragile x synd 00:02:21 [adam] ELISA for screenin HIV 00:02:31 [acestep1] cuz u cant do pcr in it 00:02:40 [Roxanita] Yamini about your question i missed the answer...the Blot tech that doesn't need Gel electrophoresis 00:02:47 [adam] yes, I got u sana, thanks 00:02:54 [Step_1] good adam. if ELISA is pos, what next? 00:03:04 [adam] it was the dot blot Rox 00:03:12 [adam] Western Blot 00:03:23 [yamini] WESTERN BLOT 00:03:32 [Step_1] right adam 00:03:34 [adam] ELISA is a very sensitive test 95% but a non specific test 00:03:51 [Roxanita] the previous answer was western blot? 00:03:56 [yamini] DOT BLOT ROXANITA 00:04:01 [adam] what is Western was equivocal? 00:04:20 [Step_1] if ELISA is pos, what next? the ans was western blot 00:04:22 [adam] sorry, what if western was equivocal? 00:04:25 [Roxanita] the Blot tech that doesn't need Gel electrophoresis? 00:04:44 [adam] It is dot blot Rox 00:04:45 [yamini] YES,ROXANITA 00:04:55 [Roxanita] dot 00:05:11 [adam] yes Rox 00:05:13 [Roxanita] uhm 00:05:31 [adam] what Rox? 00:05:34 [Step_1] the blot that doesnt require gel electo? the ans was dot blot 00:05:41 [Roxanita] I was sure it was northern blot 00:05:57 [Step_1] for which question 00:06:14 [Roxanita] that Q 00:06:24 [Roxanita] the blot that doesnt require gel electo? the ans was dot blot 00:06:40 [Roxanita] what is DOT BLOT? 00:06:50 [adam] that was my q rox 00:06:53 [acestep1] yes rox dotblot is teh only 1 tht doesnt need it 00:07:04 [Step_1] yes, the ref is k.aplan biochem genetic testing area 00:07:07 [adam] u are askin the exact same q i asked 00:07:22 [Roxanita] I even have a graphic with those.... Southern, Western and Southwestern do need 00:07:35 [Roxanita] ut Northern Blot doesn't 00:08:03 [sana123] a male child is born to a pair in which father was suffering from Leber hereditary neuropathy, what r chances that this boy will have the same disease 00:08:04 [Step_1] yes, they all need them. i dont know about northern because its not on my list 00:08:11 [acestep1] noooooooo rox dotblot doesnt need it 00:08:48 [Roxanita] DOT BLOX, page of k-aplan please 00:08:52 [Step_1] nevermind, i found it, northern does require electophoresis on my list in k.aplan 00:09:00 [yamini] NORTHERN ALSO REQUIRES ELECTROPHOROSIS 00:09:02 [adam] No rox, it does require gel electrophoresis. ref: lippincott's page 411 00:09:06 [acestep1] i dont remb but chapter 6 00:09:22 [acestep1] of <a target=new href="http://click.linksynergy.com/fs-bin/click?id=c97WUMRO5hY&offerid=47491.10000058&type=3 &subid=0" >Kaplan</a> 00:09:34 [Roxanita] I will review that because it was an N question 00:09:37 [acestep1] oops caplan imean 00:09:42 [adam] sana: Nill=zero 00:09:54 [acestep1] i think none- sana 00:09:59 [yamini] SANA THE ANS IS NONE 00:10:07 [acestep1] yes is it mitochondrial 00:10:13 [Roxanita] thanks for the question yamini 00:10:36 [adam] sana, those qs are really good 00:10:40 [yamini] U R WELCOME ROXANITA 00:10:40 [adam] thanks Sana 00:11:11 [adam] OMG, the N word!!!!!!!!!!!!!!11 00:11:14 [Step_1] none because sperm do not have mitochondria and its mitochond inheritance so father cannot pass it on 00:11:27 [sana123] yes because we inherit mitochondrail DNA only from mother,both sexes can be affected 00:11:28 [adam] EYES OPENED WIDE 00:11:37 [acestep1] yes gd explanation step1 00:11:56 [adam] nope, be careful 00:11:57 [acestep1] thnx sana 00:12:02 [adam] sperm do have mitochondria 00:12:27 [Step_1] which testing to amplify DNA? 00:12:28 [adam] other wise, how do they move? but the mitochondria is lost once the sperm enters the ovum 00:12:30 [yamini] I agree with adam 00:12:32 [adam] PCR 00:12:43 [acestep1] hey but during fertilization the tail n mitochondria r lost adam 00:12:46 [yamini] PCR 00:12:59 [acestep1] yes agree with adam 00:13:08 [Step_1] good point adam. and yes its PCR. 00:13:09 [Roxanita] good acestep1 00:13:10 [acestep1] pcr 00:13:11 [adam] that is what i said ace 00:13:27 [acestep1] thnx rox 00:13:35 [Roxanita] you guys have good information 00:13:48 [acestep1] sorry adam i ur ans came on my screen after i had typed it 00:13:59 [adam] we are learning 00:14:02 [adam] its okay ace 00:14:11 [adam] Rox, shoot! 00:14:17 [acestep1] k . thnx 00:14:37 [Step_1] which testing for small, old dried sample (like old blood)? 00:14:39 [acestep1] yes plz 00:14:40 [adam] Sana, where is ur Qs? 00:14:47 sana123 exits from this room 00:15:00 [acestep1] wow step1 cool 00:15:04 sana123 enters this room 00:15:38 [adam] hummmmmmm! is it Southern blot? 00:15:43 [acestep1] i think either mini or micro satlellite 00:15:59 [acestep1] n yes soutern blot 00:16:03 [sana123] i am sorry i couldn't get adam what exactly u asked 00:16:06 [Roxanita] person with XerodermaPigmentosum:A)is missing the enzyme that reverses thymine dimers B.is sensitive to UV radiation C. is sensitive primarily to ionizing radiation (x-rays) D.is prone to cancers and should be treated prophylactically with radiation therapy E. is missing one of the 7 genes that enc 00:16:10 [acestep1] but not sure abt teh latter 00:16:16 [Step_1] the answer would be PCR for genetic finger printing....real test question 00:16:16 [Roxanita] that encode type II topoisomerases 00:16:17 [adam] what is minni and microsatellite? 00:16:28 [adam] nothing Sana, for more Qs? 00:16:35 [acestep1] ic 00:17:08 [yamini] B 00:17:18 [acestep1] i think a rox 00:17:35 [Step_1] sens to UV radiation 00:17:51 [adam] B 00:18:05 [adam] anser Rox?! 00:18:08 [acestep1] hmm adam these r the areas of a specific # of bases which u amplify 00:18:14 [Roxanita] that means B 00:18:21 [Roxanita] Good job guys 00:18:54 [acestep1] hey wait y not a 00:19:07 [adam] so, what was the answer to ur q step 1? 00:19:34 [Step_1] the answer would be PCR for genetic finger printing....real test question 00:19:37 [adam] ace, nothing called an enzyme that reverses thymidine dimers 00:19:43 [Roxanita] In which disorder is mismatch repair found to be defective? A. Ataxia telangiectasia B. Cockayne's Syndrome C. Bloom's syndrome D. Hereditary non-polyposis colon cancer HNPCC) E. Xeroderma pigmentosum (XP) 00:20:05 [acestep1] ok yes . thnx adam 00:20:22 [Step_1] that was for the dried blood question that the real test mentioned about a forensic lab study for a murder case or something 00:20:25 [adam] thanks stpe 1 00:20:51 [acestep1] k thnx step1 00:21:00 [sana123] its D 00:21:05 [acestep1] d- rox 00:21:21 [Step_1] HNPCC 00:21:31 [yamini] D 00:21:53 [adam] are these N Qs rox? 00:22:02 [Roxanita] D) Again great! Yes, these disorders are associated with mutations in homologs of the bacterial MutS and MutL genes. 00:22:15 [sana123] so what do we use for testing old samples , step 1 plz tel 00:22:32 [adam] sana, PCR 00:22:36 [Roxanita] nope, but we got to practice from somewhere 00:22:41 [sana123] is it pcr 00:22:50 [sana123] thnaks adam 00:22:57 [adam] sure 00:22:59 [adam] thanks Rox 00:22:59 [Roxanita] I will mention N when the time comes 00:23:07 [Step_1] yes pcr for genetic finger printing 00:23:13 [adam] OK rox 00:23:26 [Roxanita] PCR yup 00:23:48 [adam] PCR is the best in the west! 00:24:03 [acestep1] lol 00:24:08 [Roxanita] let me see... 00:24:12 [adam] sorry guys, just being silly sometimes! 00:24:54 [Step_1] lol 00:24:59 [adam] okay, can you see any thing Rox? 00:25:05 [acestep1] hey adam wht u said is true . pcr si common in west 00:25:15 [sana123] Dna repair is done in which phase of cycle G1, G2, OR S 00:25:22 [acestep1] 00:25:35 [adam] G1 sana 00:25:36 [yamini] G1 00:25:36 [acestep1] g2 00:25:44 [adam] before the dudes enter S 00:25:47 [Step_1] G2 00:26:13 [Step_1] we just went over that and i already forgot 00:26:27 [Step_1] i know it starts with a G 00:26:29 [sana123] Right ans is G1, mismacth repair occurs in G 2 00:26:41 [yamini] IN G2 REPAIR OF MISMATCH PAIRS TAKES PLACE 00:26:59 [adam] great Yamini 00:27:13 [adam] Great Sana 00:27:26 [adam] I love you all guys, you are the best of the rest 00:27:34 [Step_1] yep....thats exactly what they told me when I got them mixed up a couple of hours ago....hopefully 3rd time is a charm 00:27:45 [Roxanita] N question..........The primary structural protein of the mitotic spindle apparatus is 00:28:03 [adam] Tubulin 00:28:04 [Roxanita] A) actin B) desmin C ) myosin D) tubulin E ) vimentin 00:28:09 [adam] am i right 00:28:15 [adam] heheheheh 00:28:16 [adam] D 00:28:24 [sana123] i think D 00:28:33 [Roxanita] I would mark that too, remember is "N" 00:28:37 [Step_1] i'd say tubulin 00:28:55 [adam] ok, Hoshhhhhhhhhhhhhhhhhh, No N's or M's or F's 00:29:12 [Step_1] how about N = No answer provided 00:29:13 [adam] shoot Rox, You are killing me! 00:29:39 [sana123] HNPCC is a mismatch repair defect or DNA repair defect 00:29:40 [Step_1] lol 00:29:56 [Step_1] mismatch 00:30:33 [Step_1] wait, its a mismatch dna repair defect...trick question 00:30:56 [sana123] yes step 1 it is 00:30:58 [acestep1] agree with step1 00:31:02 [adam] i need to reread this 00:31:11 [Roxanita] I guess I wont kill you anymore cause I am tired 00:31:16 [Roxanita] 00:31:28 [adam] no, i was just kidding you Rox 00:31:34 [adam] you are doing great 00:31:37 [acestep1] 00:31:40 [Roxanita] ok Guys I will be posting new questions and remember the N questions don't have answers, 00:32:04 [acestep1] k 00:32:09 [Step_1] ok, i guess we should call it a night...or day depending on location 00:32:15 [Roxanita] we are behind, next chat will be criminal for us, we have so many chapters to do on saturday 00:32:17 [adam] now? or on the forum Rox? 00:32:43 [Roxanita] on the forum my new partner 00:32:48 [acestep1] yes 00:32:55 [Step_1] we can try to knock some material out in the step 1 forum in between 00:32:59 [acestep1] 00:33:10 [Roxanita] so for next chat saturday night, we are supposed to cover everything 00:33:11 [acestep1] yes sure 00:33:17 [adam] Okay great Rox. I go quite ofetrn to usmlenet. I will move to ur great forum 00:33:19 [adam] you are the best 00:33:30 [Roxanita] right Step1, again thanks for your great replies and pictures 00:33:33 [adam] every thing in What great Rox? 00:33:44 [Step_1] everything except genetics i think 00:33:45 [sana123] what is MOA OF ETOPOSIDE AND TENIPOSIDE a.it inhibits thymidylate synthetase, b; inhibits top 2, c; inhibits reverse transcriptase 00:33:48 [acestep1] 00:34:07 [adam] Top2 00:34:11 [acestep1] bn 00:34:12 [adam] answer is b 00:34:16 [yamini] TOP2 00:34:17 [Roxanita] in the forum go to SCHEDULE FOR CHAT GROUP is on announcements 00:34:20 [acestep1] b 00:34:40 [adam] what is the mode of action of Topotecan? 00:34:48 [acestep1] oh ok so genetics step1 ? 00:34:53 [Roxanita] wow sana still alive I have nistagmus right now, got to go 00:35:11 [Step_1] inhibits the activity of topoisomerase II, 00:35:13 [acestep1] hey adam wht is tht 00:35:20 [acestep1] lol 00:35:33 [adam] see?! 00:35:34 [yamini] WHAT IS THE ANS ADAM? 00:35:38 [adam] it is a drug 00:35:41 [adam] a new one 00:35:51 [acestep1] ic 00:35:54 [Roxanita] this remind me the musicians at TITANIC never quit 00:35:55 [Step_1] no genetics or very briefly because we already discussed it a couple of weeks ago 00:35:56 [adam] It inhibits Topoisomerase I 00:36:02 [acestep1] thnx both step1 n adam 00:36:07 [sana123] i am on west coast so its not too late,besides u guys have done very good job for us 00:36:12 [adam] yes Rox, stay like the rocks 00:36:18 [acestep1] k 00:36:30 [sana123] its topo 2 inhibitor 00:36:33 [adam] what is the mode of resistance to etoposide? 00:36:39 [Step_1] i'm on the east coast and have to get up pretty early 00:36:57 [acestep1] 00:37:23 [Roxanita] I am in chicago, here it's 11:37pm 00:37:27 [acestep1] u change the topoiso 2 ? 00:37:41 [adam] Rox, I am in chicago too 00:37:48 [acestep1] ok im in asia n here its morning. lol 00:37:56 [Step_1] 12:37 here and have to be up at 7 00:38:02 [adam] we are neighbors and you don't say a word? hummmmmmmmmm! 00:38:12 [acestep1] lol 00:38:30 [acestep1] hey i think b should call it a day / nite 00:38:37 [Roxanita] oh oh, then I am in wisconsin 00:38:40 [acestep1] n post ur qs on teh forum 00:38:48 [adam] no ace, this is a method of resistance in bacteria 00:38:48 [Roxanita] Just kidding 00:38:51 [acestep1] lol 00:38:54 [Step_1] 00:39:16 [yamini] P-GLYCOPROTEIN/MUTATION OF THE ENZYME ADAM 00:39:20 [adam] wow, I am moving tomorrow morning to Wisconsin, what a small world 00:39:26 [acestep1] ic 00:39:29 [adam] excellent Yamini 00:39:38 [adam] this is a very important question 00:39:45 [acestep1] lol 00:39:50 [adam] you know what other drugs share the same mechanism of resistance? 00:39:51 [acestep1] k 00:39:55 [sana123] buy the way, etoposide is eukaryotic topo 2 inhibitor,nalidixic acid a quinione is prokaryotic so resistance should be a problem with quinolones, or what u guys say 00:39:58 [Roxanita] 00:40:06 [Step_1] ok, goodnight everyone. if you guys continue when i leave, please post the remainder of the chat that i missed so everyone can read it 00:40:19 [Roxanita] how do you post it. 00:40:40 [adam] by mail rox! 00:40:42 [acestep1] gn step1 . take care 00:41:01 [adam] gn step 1 00:41:05 [adam] thanks alot of r ur time 00:41:10 [Step_1] i will post up until this point....actually.....dont worry, i'll just leave the computer running and get the rest when i get up later. 00:41:11 [sana123] good night step 1,good luck 00:41:20 [Roxanita] I mean once is gone I don't know how to save the remain chat 00:41:46 [adam] hummmmm. I donno Rox 00:41:51 [acestep1] k . thnx step1 00:42:06 [Roxanita] Thanks step 1, sleep good 00:42:14 [Step_1] dont worry rox, i'll leave it running so we wont miss anything. thanks everyone. bye 00:42:14 [adam] Yamini , did u get to know what other drugs share the same mech of resistance? 00:42:46 [yamini] ALKYLATING AGENTS 00:42:47 [acestep1] ur welcoem step1 byee 00:42:54 [adam] leave who running! we are not running, we are chatting 00:43:05 [adam] take this Yammini 00:43:22 [acestep1] lol 00:43:23 [Roxanita] ok guys now is the end for me. Great to see everybody: acestep1 adam, hutals, julieog1, nne, sana123, step1 my leading strand, and Yamini of course 00:43:38 [adam] Protein P Drives Drug Efflux Very Badly 00:43:48 [adam] P: Paclitaxle 00:44:01 [acestep1] hey lorena take care 00:44:08 [sana123] plz elaorate for us,the mechanismof resistance who knows it well, i am really weak in this 00:44:12 [Roxanita] See you guys on saturday and answer to my questions ok THANKS A LOT GUYS 00:44:12 [adam] D: Duxorubiocin 00:44:18 [acestep1] oops imean rox 00:44:20 [adam] D: Dactinomicin 00:44:31 [Roxanita] Lorena I feel so appreciated 00:44:33 [adam] V: Vincristine and Vinblastine 00:44:38 [adam] B: B;eomycin 00:44:42 [acestep1] hehe 00:44:45 [adam] Bleomycin sorry 00:44:52 [acestep1] my head is pinning 00:45:00 [adam] E: Etoposide 00:45:03 [acestep1] spinning imean 00:45:04 [yamini] ROXANITA GOD NIGHT 00:45:07 [Roxanita] Ciao chicos. 00:45:14 [adam] Roxanita 00:45:21 [acestep1] 00:45:33 [adam] Good Night. see ya in Wisconsin tomorrow mornin 00:45:37 [acestep1] hey guys i gtg 2 00:45:38 [sana123] good night rox 00:45:49 [Roxanita] greetings to Eva, Adam, bye. 00:45:50 [acestep1] lol 00:46:02 [adam] Rox, where in chicago are ya? 00:46:05 [acestep1] byeeeeeee 00:46:15 [julieog1] bye 00:46:21 [sana123] adam plz tell, do u mean that |
