Anonymous
06-04-2004, 09:57 PM
Problem 10 Recombinant DNA 5
One of the most significant discoveries which allowed the development of recombinant DNA technology was:
A. the discovery of antibiotics used for selecting transformed bacteria.
B. the identification and isolation of restriction endonucleases permitting specific DNA cutting.
C. the discovery of DNA and RNA polymerase allowing workers to synthesize any DNA sequence.
D. the development of the polymerase chain reaction.
E. the Southern technique for separation and identification of DNA sequences.
Restriction endonucleases
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/10t.gif
Restriction endonucleases -- cut double stranded DNA at specific sequences, protection against viruses in bacteria.
Sequences may be palindromes: a sequence which is the same when read in either direction. "Able was I ere I saw Elba"
Example of specificity of restriction endonucleases
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/10ta.gif
Restriction endonucleases allow the specific and reproducible fragmentation of DNA.
The discovery of these enzymes allowed the development of modern recombinant DNA technology.
ans:
B. the identification and isolation of restriction endonucleases permitting specific DNA cutting.
Prior to the discovery of these enzymes, there was no reproducible way to fragment DNA.
One of the most significant discoveries which allowed the development of recombinant DNA technology was:
A. the discovery of antibiotics used for selecting transformed bacteria.
B. the identification and isolation of restriction endonucleases permitting specific DNA cutting.
C. the discovery of DNA and RNA polymerase allowing workers to synthesize any DNA sequence.
D. the development of the polymerase chain reaction.
E. the Southern technique for separation and identification of DNA sequences.
Restriction endonucleases
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/10t.gif
Restriction endonucleases -- cut double stranded DNA at specific sequences, protection against viruses in bacteria.
Sequences may be palindromes: a sequence which is the same when read in either direction. "Able was I ere I saw Elba"
Example of specificity of restriction endonucleases
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/10ta.gif
Restriction endonucleases allow the specific and reproducible fragmentation of DNA.
The discovery of these enzymes allowed the development of modern recombinant DNA technology.
ans:
B. the identification and isolation of restriction endonucleases permitting specific DNA cutting.
Prior to the discovery of these enzymes, there was no reproducible way to fragment DNA.