PDA

View Full Version : Problem 9 Recombinant DNA 4


Anonymous
06-04-2004, 09:55 PM
"Gene library" is a term used to describe:


A. a computerized listing of known DNA sequences .
B. bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal.
C. a collection of books about recombinant DNA technology.
D. a compilation of the amino acid sequences of protein coding genes.
E. a store that specializes in the sale of Levis.




Gene library

When the genomic DNA is digested by a restriction endonuclease, and all fragments cloned at random into a plasmid vector, then the majority of genetic information will be included in the mixture of bacteria.
Cultures of the bacteria, with each containing only a fraction of the genome, collectively contain all the genes and are called a library.

http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/04t.gif



ans:
B. bacteria with plasmids containing DNA fragments representing the majority of the genetic information from a plant or animal.

A library is a culture of bacteria where each cell has one or a few DNA sequences from another organism, but the whole culture contains the majority of the DNA fragments from an organism.