Anonymous
06-04-2004, 09:35 PM
Problem 6 Applications
Restriction endonuclease generated DNA fragments separated by gel electrophoresis and blot transfered onto a membrane filter are probed with a radioactive DNA fragment. This procedure is called:
A. Gene cloning
B. The Southern technique
C. The polymerase chain reaction
D. Recombinant DNA
E. Gene mapping
The Southern Technique
Transfering DNA fragments to nitrocellulose sheets
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/03tb.gif
DNA fragments are transfered to nitrocellulose sheets where they bind.
DNA fragments are denatured and separated by gel electrophoresis
Fragments are blotted onto a sheet of nitrocellulose and fixed by heating
Blot is reacted with a radioactive probe of RNA or DNA which binds to complementary DNA
Autoradiography is used to detect radioactive fragments
Fixing the fragments of DNA
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/03tc.gif
The denatures fragments of DNA are fixed by baking.
A radioactive probe is added. It can hybridize with a gene sequence in the DNA.
The sheet is rinsed and placed next to X-ray film for autoradiography.
ans:
B. The Southern technique
The Southern technique detects the location of DNA fragments on gel electrophoresis
Restriction endonuclease generated DNA fragments separated by gel electrophoresis and blot transfered onto a membrane filter are probed with a radioactive DNA fragment. This procedure is called:
A. Gene cloning
B. The Southern technique
C. The polymerase chain reaction
D. Recombinant DNA
E. Gene mapping
The Southern Technique
Transfering DNA fragments to nitrocellulose sheets
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/03tb.gif
DNA fragments are transfered to nitrocellulose sheets where they bind.
DNA fragments are denatured and separated by gel electrophoresis
Fragments are blotted onto a sheet of nitrocellulose and fixed by heating
Blot is reacted with a radioactive probe of RNA or DNA which binds to complementary DNA
Autoradiography is used to detect radioactive fragments
Fixing the fragments of DNA
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/03tc.gif
The denatures fragments of DNA are fixed by baking.
A radioactive probe is added. It can hybridize with a gene sequence in the DNA.
The sheet is rinsed and placed next to X-ray film for autoradiography.
ans:
B. The Southern technique
The Southern technique detects the location of DNA fragments on gel electrophoresis