Anonymous
06-04-2004, 09:24 PM
Problem 4 Recombinant DNA 2
DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is refered to as a:
A. restriction map
B. RFLP profile
C. F' factor
D. library
E. lysogenic phage
Gene library
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/04ta.gif
When the genomic DNA is digested by a restriction endonuclease, and all fragments cloned at random into a plasmid vector, then the majority of genetic information will be included in the mixture of bacteria.
Cultures of the bacteria, with each containing only a fraction of the genome, collectively contain all the genes and are called a library.
ans:
D. library
A library is a culture of bacteria where each cell has one or a few DNA sequences from another organism, but the whole culture contains the majority of the DNA fragments from an organism.
DNA from a eukaryotic organism is digested with a restriction endonuclease and the resulting fragments cloned into a plasmid vector. Bacteria transformed by these plasmids collectively contain all of the genes of the organism. This culture of bacteria is refered to as a:
A. restriction map
B. RFLP profile
C. F' factor
D. library
E. lysogenic phage
Gene library
http://www.biology.arizona.edu/molecular_bio/problem_sets/Recombinant_DNA_Technology/graphics/04ta.gif
When the genomic DNA is digested by a restriction endonuclease, and all fragments cloned at random into a plasmid vector, then the majority of genetic information will be included in the mixture of bacteria.
Cultures of the bacteria, with each containing only a fraction of the genome, collectively contain all the genes and are called a library.
ans:
D. library
A library is a culture of bacteria where each cell has one or a few DNA sequences from another organism, but the whole culture contains the majority of the DNA fragments from an organism.