Anonymous
06-04-2004, 09:07 PM
Problem 1 PCR primers
Primers
The process of DNA replication uses RNA primers. To make a copy of DNA from a known part of the chromosome, for example by PCR, short primers of DNA are added to DNA in a test tube and the appropriate enzymes are included to make a copy of the DNA. The primers must be complementary to the DNA and obey the polarity rule.
For the sequence: 5' ATGCCTAGGTC the primer would have to be 5'GACCT. Extending the 3' T would copy the single stranded DNA sequence.
ans:
D. 5' GACCT
The 3' end of the template strand starts the 5' of the newly synthesized DNA.
Primers
The process of DNA replication uses RNA primers. To make a copy of DNA from a known part of the chromosome, for example by PCR, short primers of DNA are added to DNA in a test tube and the appropriate enzymes are included to make a copy of the DNA. The primers must be complementary to the DNA and obey the polarity rule.
For the sequence: 5' ATGCCTAGGTC the primer would have to be 5'GACCT. Extending the 3' T would copy the single stranded DNA sequence.
ans:
D. 5' GACCT
The 3' end of the template strand starts the 5' of the newly synthesized DNA.