DNA pol I vs. DNA pol III

[Ella]

Ok could someone please explain to me the difference between DNA pol I and DNA pol III. They both have exonuclease activity but I can't seem to understand what each is used for and the directions.

Thanks!

[Dr. X]

is this question still being asked after pg. 85 of FA?

[5star]

hi, can any1 plz explain me what is expression cloning??? and few examples.... awaiting a quick reply thanx!

[Ella]

is this question still being asked after pg. 85 of FA?


Hmm...Dr. X - what FA edition are you refering to? I amusing 2005 ed and 2006 ed!

[sunshine81]

DNA Polymerase III - makes the chain in a 5 to 3 direction and it proofreads with a 3 to 5 exonuclease, so it goes both ways doing something different
DNA Polymerase I - breaks down the RNA primer with a 5 to 3 exonuclease

I got that straight out of the FA, if the pages don't match up just look up DNA polymerase in the index

[Ella]

See that is exactly what I thought as well...but there is a questions I got in UW and in there the explaination was different which totally confused me. I think in the UW explaination they went more in depth than just the one small section in FA.

Either way, it's better to just keep it simple! I didn't like UW's explaination.

Thanks!