molecular biochemistry

[sarda]

hi
help me with this q
12 base nucleotide sequence likely to use as probe complementery to coding strand of sickle cell allele
1)CCTCACCTGCGG
2)CTTCTCCACAGG

[buffy coat]

none of these.


answer will be: CTCCTGTGGAGAAGT as a probe for coding strand GAGGACACCTCTTCAGACG of HbS mRNA.

[sarda]

thanks .please explan.i am poor in this thing

[MDiva]

Can anyone explain this?

[Kite]

hi
help me with this q
12 base nucleotide sequence likely to use as probe complementery to coding strand of sickle cell allele
1)CCTCACCTGCGG
2)CTTCTCCACAGG

Ill take a shot at it...

nucleotides are coded for in triplet sequences.. which means that for every three nucleotides (which is one codon sequence..), 1 amino acid is formed. This is becuase DNA is degenerate, it means that multiple codon sequences can code for the same amino acid.

The problem HbS hemoglobin, or sickle cell is that in the coding sequence, glutamate is replaced with valine. You can find a chart for coding amino acids in any biochemistry book I imagine.. Im currently using Harper's biochem.

The one of the coding sequences for Valine just happens to be GTG


My final conclusion would be that the answer is all of the above...

CCTCACCTGCGG
GGAGTGGACGCC

CTTCTCCACAGG
GAAGAGGTGTCC

Hence they can both be used as probes for the complementery strand that codes for sickle cell.

Anyone else have anything to add? Thats my conclusion to the best of my logic and information, But someone definitely add to this because its has struck my curiosity too!!!